Active Recombinant Cynomolgus SOST protein, His-tagged

• Cat.No.: SOST-0724C
• Product Overview: Recombinant Cynomolgus SOST protein(Gln24-Tyr213), fused with His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Cynomolgus
• Tag: His
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
• Bio-activity: Immobilized Cynomolgus SOST, His Tag at 5 μg/mL (100 μL/well) can bind Biotinylated Human LRP-6, His,Avitag with a linear range of 0.005-0.156 μg/mL. Immobilized Cynomolgus SOST, His Tag at 5 μg/mL (100 μL/well) can bind Biotinylated Human LRP-5, His,Avitag with a linear range of 0.01-0.313 μg/mL. Immobilized Cynomolgus SOST, His Tag at 5 μg/mL (100 μL/well) can bind Human LRP-5, Mouse IgG2a Fc Tag with a linear range of 0.005-0.313 μg/mL.
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminusThe protein has a calculated MW of 23.4 kDa. The protein migrates as 31-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Gln24-Tyr213
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Protein length: 24-213 a.a.