Active Recombinant Human SMPD1 protein, His-tagged

• Cat.No.: SMPD1-537H
• Product Overview: Recombinant Human SMPD1 protein(NP_000534.3)(Met1-Pro628), fused with His tag, was expressed in Insect Cells.

Specification

• Source: Insect Cells
• Species: Human
• Tag: His
• Form: Supplied as sterile 20 mM Tris, 500 mM NaCl, 25 % glycerol, pH 7.5.Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: Measured by its ability to cleave 2-N-Hexadecanoylamino-4-nitrophenylphosphorylcholine (HNPPC). The specific activity is >1000 pmol/min/μg.
• Molecular Mass: The recombinant human SMPD1 consists of 593 amino acids and predicts a molecular mass of 66.3 kDa.
• Protein length: Met1-Pro628
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: SMPD1 sphingomyelin phosphodiesterase 1, acid lysosomal [ Homo sapiens ]
• Official Symbol: SMPD1
• Synonyms: SMPD1; sphingomyelin phosphodiesterase 1, acid lysosomal; sphingomyelin phosphodiesterase; acid sphingomyelinase; ASM; NPD; ASMASE
• Gene ID: 6609
• mRNA Refseq: NM_000543
• Protein Refseq: NP_000534
• MIM: 607608
• UniProt ID: P17405