Active Recombinant Mouse Smpd1 protein(Met1-Leu626), His-tagged

• Cat.No.: Smpd1-2552M
• Product Overview: Recombinant Mouse SMPD1 (Q04519) (Met 1-Leu 626) was expressed in Insect Cells,with a C-terminal polyhistidine tag.

Specification

• Species: Mouse
• Source: Insect Cells
• Tag: His
• Protein Length: Met1-Leu626
• Form: Supplied as sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 8.0, 0.1% Tween20.
• Bio-activity: Measured by its ability to cleave 2-N-Hexadecanoylamino-4-nitrophenylphosphorylcholine(HNPPC). The specific activity is > 1, 000 pmoles/min/μg.
• Molecular Mass: The secreted recombinant mouse SMPD1 consists of 592 amino acids and has a calculated molecular mass of 66.3 kDa. It migrates as an approximately 63 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 85 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Concentration: Please refer to the hard copy of CoA.

Gene Information

• Gene Name: Smpd1 sphingomyelin phosphodiesterase 1, acid lysosomal [ Mus musculus ]
• Official Symbol: Smpd1
• Synonyms: SMPD1; sphingomyelin phosphodiesterase 1, acid lysosomal; sphingomyelin phosphodiesterase; acid sphingomyelinase; ASM; aSMase; A-SMase; Zn-SMase
• Gene ID: 20597
• mRNA Refseq: NM_011421
• Protein Refseq: NP_035551