Active Recombinant Human Slit1, His-tagged

• Cat.No.: SLIT1-19H
• Product Overview: Recombinant Human Slit1, expressed in CHO.

Specification

• Species: Human
• Source: CHO
• Tag: His
• Description: Slit1 is a member of the Slit family of large secreted axon guidance molecules that are ligands for Robo receptors. Like other mammalian family members, the 1531 amino acid (aa), ~200 kDa Slit1 contains a 33 aa signal sequence followed by 23 leucine rich repeats (LRR, aa 34 900) and 9 EGF like sequences (aa 930 1451). Mammalian Slits also contain a laminin G domain between EGF6 and EGF7 (aa 1163 1336), and a C-terminal cysteine rich domain (cysteine knot? aa 1456 1531). Heparin sulfates are required for interaction of Robo with Slit LRR domains. Mouse Slit1 shares 99, 96, 89, 87 and 80% aa identity with rat, human, canine, Xenopus and zebrafish Slit1, respectively, within the LRR domains. Mouse Slits 1, 2 and 3 share 68 74% aa identity within the LRR domains. Slit1 and Slit2 (or in some cases Slit3) are expressed in complementary l centigradeations during development of the optic and olfactory tracts and the forebrain, and appear to work together to mediate Robo guidance of retinal, olfactory, hipp centigradeampal and motor axons.
• Form: Lyophilized from a 0.2 μm filtered solution in MOPS and NaCl. See Certificate of Analysis for details.
• Bio-activity: Measured by its ability to enhance neurite outgrowth of diss centigradeiated E13 chick embryonic dorsal root ganglia (DRG) neurons. Able to significantly enhance neurite outgrowth when immobilized as a 3 μL droplet containing 90 ng on a nitr centigradeellulose coated microplate.
• Molecular Mass: Predicted molecular mass: 97.6 kDa SDS PAGE: 110 kDa, under reducing conditions
• Endotoxin: <1.0 eu per 1 μg of the protein by the lal</1.0
• Purity: >80%, by SDSPAGE under reducing conditions and visualized by silver stain.
• Usage: FOR RESEARCH USE ONLY
• Quality Control Test: 12 months from date of receipt, 20 to 70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, 20 to 70 centigrade under sterile conditions after reconstitution.
• Reconstitution: Reconstitute at 200 μg/mL in sterile PBS.
• Warning: Avoid repeated freeze-thaw cycles.

Gene Information

• Gene Name: SLIT1 slit homolog 1 (Drosophila) [ Homo sapiens ]
• Official Symbol: SLIT1
• Synonyms: SLIT1; slit homolog 1 (Drosophila); SLIL1, slit (Drosophila) homolog 1; slit homolog 1 protein; MEGF4; Slit 1; slit1; SLIT3; multiple EGF-like domains protein 4; multiple epidermal growth factor-like domains protein 4; SLIL1; SLIT-1; MGC164811
• Gene ID: 6585
• mRNA Refseq: NM_003061
• Protein Refseq: NP_003052
• MIM: 603742
• UniProt ID: O75093
• Chromosome Location: 10q23.3-q24
• Pathway: Axon guidance, organism-specific biosystem; Axon guidance, conserved biosystem; Axon guidance, organism-specific biosystem; Developmental Biology, organism-specific biosystem; Netrin-1 signaling, organism-specific biosystem
• Function: Roundabout binding; calcium ion binding; heparan sulfate proteoglycan binding