Active Recombinant Human SETD2 protein, GST-tagged

• Cat.No.: SETD2-199H
• Product Overview: Recombinant Human SETD2(1425-1717 aa) fused with GST tag at N-terminal was expressed in Insect cells.

Specification

• Species: Human
• Source: Insect Cells
• Tag: GST
• Protein Length: 1425-1717 a.a.
• Description: As a member of the mixed lineage leukemia (MLL) family of histone methyltransferase, SETD2 specifically tri-methylates 'Lys-36' of histone H3 (H3K36me3) using dimethylated 'Lys-36' (H3K36me2) as substrate. The main enzyme generating H3K36me3, a specific tag for epigenetic transcriptional activation.
• Form: 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Bio-activity: 250 nmol/min/mg
• Molecular Mass: 61 kDa
• Purity: >70%
• Applications: Methyltransferase Assay
• Stability: 1 year at -70 centigrade from the date of shipment
• Storage: Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.1μg/μl

Gene Information

• Gene Name: SETD2 SET domain containing 2 [ Homo sapiens ]
• Official Symbol: SETD2
• Synonyms: SETD2; SET domain containing 2; histone-lysine N-methyltransferase SETD2; FLJ23184; HIF 1; HYPB; KIAA1732; KMT3A; huntingtin yeast partner B; lysine N-methyltransferase 3A; huntingtin interacting protein 1; huntingtin-interacting protein B; SET2; HIF-1; H
• Gene ID: 29072
• mRNA Refseq: NM_014159
• Protein Refseq: NP_054878
• MIM: 612778
• UniProt ID: Q9BYW2
• Chromosome Location: 3p21.31
• Pathway: Lysine degradation, organism-specific biosystem; Lysine degradation, conserved biosystem
• Function: DNA binding; histone-lysine N-methyltransferase activity; methyltransferase activity; oxidoreductase activity; protein binding; transferase activity; transition metal ion binding