Active Recombinant Human SERPINI1 Protein, GST-tagged

• Cat.No.: SERPINI1-01H
• Product Overview: Recombinant Human SERPINI1 (Thr17-Leu410) protein without tag was expressed in CHO.

Specification

• Species: Human
• Source: CHO
• Protein Length: 17-410 a.a.
• Description: This gene encodes a member of the serpin superfamily of serine proteinase inhibitors. The protein is primarily secreted by axons in the brain, and preferentially reacts with and inhibits tissue-type plasminogen activator. It is thought to play a role in the regulation of axonal growth and the development of synaptic plasticity. Mutations in this gene result in familial encephalopathy with neuroserpin inclusion bodies (FENIB), which is a dominantly inherited form of familial encephalopathy and epilepsy characterized by the accumulation of mutant neuroserpin polymers. Multiple alternatively spliced variants, encoding the same protein, have been identified.
• Bio-activity: ED50 < 2.0 μg/mL, measured by the dose-dependent stimulation of the proliferation of rat C6 cells, corresponding to a specific activity of > 500.0 units/mg.
• Molecular Mass: 40~45 kDa, on SDS-PAGE under non-reducing conditions.
• Endotoxin: < 0.2 EU/ μg of protein by gel clotting method
• Purity: > 95% as analyzed by SDS-PAGE
  > 95% as analyzed by HPLC
• Storage: Upon receiving, this product remains stable for up to 6 months at lower than -70 centigrade. Upon reconstitution, the product should be stable for up to 1 week at 4 centigrade or up to 3 months at -20 centigrade. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Lyophilized after extensive dialysis against PBS.
• Reconstitution: It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH2O or PBS up to 100 μg/mL.

Gene Information

• Gene Name: SERPINI1 serpin family I member 1 [ Homo sapiens (human) ]
• Official Symbol: SERPINI1 serpin family I member 1 [ Homo sapiens (human) ]
• Synonyms: SERPINI1; serpin family I member 1; PI12; HNS-S1; HNS-S2; neuroserpin; neuroserpin; PI-12; peptidase inhibitor 12; serine (or cysteine) proteinase inhibitor, clade I (neuroserpin), member 1; serpin I1; serpin peptidase inhibitor clade I member 1; serpin peptidase inhibitor, clade I (neuroserpin), member 1; truncated HNS-T
• Gene ID: 5274
• mRNA Refseq: NM_005025
• Protein Refseq: NP_005016
• MIM: 602445
• UniProt ID: Q99574