Active Recombinant Human SEMA6B, Fc Chimera

• Cat.No.: SEMA6B-1925H
• Product Overview: Recombinant Human SEMA6B fussed with a Fc region of human IgG1 at the C-terminus via a peptide linker was expressed in a mouse myeloma cell line,NS0.

Specification

• Species: Human
• Source: Mammalian Cells
• Tag: Fc
• Description: This gene encodes a member of the semaphorin family, a group of proteins characterized by the presence of a conserved semaphorin (sema) domain. Whereas some semaphorins are transmembrane proteins, others are secreted. Semaphorins play a major role in axon guidance. The protein encoded by this gene may be involved in both peripheral and central nervous system development.
• Purity: > 95%, as determined by SDS-PAGE and visualized by silver stain.
• Molecular Mass: The mature recombinant human Semaphprin 6B/Fc chimera, generated by proteolytic removal of the signal peptide region, is a homodimeric protein. Based on N-terminal sequencing, the mature recombinant human Semaphorin 6B protein starts at Leu 26. The monomer of the recombinant human Semaphorin 6B has a calculated molecular mass of approximately 63.4 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 110 - 120 kDa protein in SDS-PAGE under reducing conditions.
• Activity: Measured by its ability to cause collapse of chick DRG growth cones. The ED50 for this effect is typically 2.5 - 5.0 μg/mL in the presence of 10 ng/mL of rhβ-NGF.
• Endotoxin: < 1.0 EU per 1 μg of the enzyme as determined by the LAL method.
• Format: Lyophilized from a 0.2 μm filtered solution in PBS.
• Storage: Samples are stable for up to six months at -70°C in a manual defrost freezer after the date of receipt. After receiving, this protein can be aliquoted and stored at above temperature for up to six months without a significant loss of activity. Avoid repeated freeze-thaw cycles.

Gene Information

• Gene Name: SEMA6B sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6B [ Homo sapiens ]
• Synonyms: SEMA6B; sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6B; SEMAN; semaZ; SEMA-VIB; SEM-SEMA-Y; SEMA6B; semaphorin Z; semaphorin VIB
• Gene ID: 10501
• mRNA Refseq: NM_032108
• Protein Refseq: NP_115484
• MIM: 608873
• UniProt ID: Q9H3T3
• Chromosome Location: 19p13
• Function: protein binding; protein binding; protein complex binding