Active Recombinant Human SELPLG Protein, Fc-tagged

• Cat.No.: SELPLG-242H
• Product Overview: Recombinant human SELPLG protein (Gln42-Val295) with Fc fragment of a Human IgG1 (Pro100-Lys330) at C-terminus was expressed in Chinese hamster hvary cell line.

Specification

• Source: CHO
• Species: Human
• Tag: Fc
• Predicted N Terminal: Gln42
• Form: Disulfide-linked homodimer
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of CHO Chinese hamster ovary cells transfected with P-Selectin. Recombinant Human PSGL‑1/CD162 immobilized at 3 μg/mL (100 μL/well) will induce >70% adhesion of CHO Chinese hamster ovary cells transfected with P-Selectin.
• Molecular Mass: 52.6 kDa (monomer)
• Endotoxin: <0.10 EU per 1 μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in Tris and NaCl.
• Reconstitution: Reconstitute at 100 μg/mL in sterile PBS.
• Shipping: The product is shipped at ambient temperature.
• Protein length: 42-295 a.a.

Gene Information

• Gene Name: SELPLG selectin P ligand [ Homo sapiens (human) ]
• Official Symbol: SELPLG
• Synonyms: SELPLG; selectin P ligand; CLA; CD162; PSGL1; PSGL-1; P-selectin glycoprotein ligand 1; cutaneous lymphocyte-associated associated antigen
• Gene ID: 6404
• mRNA Refseq: NM_003006
• Protein Refseq: NP_002997
• MIM: 600738
• UniProt ID: Q14242