Active Recombinant Human SELPLG protein, His-tagged

• Cat.No.: SELPLG-533H
• Product Overview: Recombinant Human SELPLG protein(Gln 42 - Cys 320), fused with His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: His
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.
• Bio-activity: Human PSGL-1, His Tag immobilized on CM5 Chip can bind Human B7-H5, Fc Tag with an affinity constant of 0.213 μM as determined in a SPR assay (Biacore T200).
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminus.The protein has a calculated MW of 30.7 kDa. The protein migrates as 55-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Protein length: Gln 42 - Cys 320
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: SELPLG selectin P ligand [ Homo sapiens ]
• Official Symbol: SELPLG
• Synonyms: SELPLG; selectin P ligand; P-selectin glycoprotein ligand 1; CD162; PSGL 1; cutaneous lymphocyte-associated associated antigen; CLA; PSGL1; PSGL-1
• Gene ID: 6404
• mRNA Refseq: NM_001206609
• Protein Refseq: NP_001193538
• MIM: 600738
• UniProt ID: Q14242