Active Recombinant Human Secreted Phosphoprotein 1, HIgG1 Fc-tagged, mutant

• Cat.No.: SPP1-120H
• Product Overview: The extracellular domain of human OPN(AAH07016.1)(Ile17-Asn300) is fused to the N-terminus of the Fc region of a mutant human IgG1 was expressed in CHO cell.

Specification

• Description: The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene.
• Source: CHO cell
• Species: Human
• Tag: HIgG1 Fc-tagged, mutant
• Form: Lyophilized from 0.2 μm-filtered solution in PBS.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of HEK293 human embryonic kidney cells.
• Molecular Mass: 58KDa (monomer)
• AA Sequence: Ile17-Asn300
• Endotoxin: <0.06 eu/μg as determined by lal test.>
• Purity: >98%, by SDS-PAGE under reducing conditions
• Stability: Stable for at least 1 year after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.
• Reconstitution: Reconstitute at 100 μg/ml in sterile PBS.
• Warning: Avoid freeze/thaw cycles.
• Protein length: Ile17-Asn300

Gene Information

• Gene Name: SPP1 secreted phosphoprotein 1 [ Homo sapiens ]
• Official Symbol: SPP1
• Synonyms: SPP1; secreted phosphoprotein 1; BNSP, bone sialoprotein I , OPN, osteopontin; osteopontin; BSPI; early T lymphocyte activation 1; ETA 1; uropontin; nephropontin; osteopontin-C; osteopontin-D; SPP1/CALPHA1 fusion; bone sialoprotein 1; urinary stone protein; early T-lymphocyte activation 1; osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein; secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1); OPN; BNSP; ETA-1; MGC110940
• Gene ID: 6696
• mRNA Refseq: NM_000582
• Protein Refseq: NP_000573
• MIM: 166490
• UniProt ID: P10451
• Chromosome Location: 4q22.1
• Pathway: Direct p53 effectors, organism-specific biosystem; ECM-receptor interaction, organism-specific biosystem; ECM-receptor interaction, conserved biosystem; Endochondral Ossification, organism-specific biosystem; FGF signaling pathway, organism-specific biosystem; Focal Adhesion, organism-specific biosystem; Focal adhesion, organism-specific biosystem
• Function: cytokine activity; extracellular matrix binding