Active Recombinant Human S100B protein(Ser2-Glu92), His-tagged

• Cat.No.: S100B-31010TH
• Product Overview: Recombinant Human S100B (NP_006263.1) (Ser 2-Glu 92) was expressed in E. coli, fused with a polyhistidine tag at the N-terminus.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: Ser2-Glu92
• Form: Lyophilized from sterile PBS, 10% glycerol, pH 7.8. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: 1. Measured by its ability to bind human AGER in a functional ELISA. 2. Measured by its ability to bind TP53 in a functional ELISA.
• Molecular Mass: The recombinant human S100B consisting of 102 amino acids and migrates as an approximately 12 kDa band in SDS-PAGE under reducing conditions as predicted.
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: S100B S100 calcium binding protein B [ Homo sapiens ]
• Official Symbol: S100B
• Synonyms: S100B; S100 calcium binding protein B; S100 calcium binding protein, beta (neural); protein S100-B; S100beta; S-100 protein subunit beta; S-100 calcium-binding protein, beta chain; S100 calcium-binding protein, beta (neural); NEF; S100; S100-B
• Gene ID: 6285
• mRNA Refseq: NM_006272
• Protein Refseq: NP_006263
• MIM: 176990
• UniProt ID: P04271