Recombinant Active Mouse ROCK2 Protein (5-554), N-His-tagged

• Cat.No.: ROCK2-37M
• Product Overview: Recombinant mouse ROCK2 (5-554) was expressed using baculovirus in Sf9 insect cells using an N-terminal His tag.

Specification

• Description: ROCK2 is a ubiquitously expressed serine/threonine kinase localized in the nucleus that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. ROCK2 is an immediate downstream target of the small GTPase RhoA. ROCK2 may play a pivotal role in cardiovascular diseases such as vasospastic angina, ischemic stroke, and heart failure. Inhibition of ROCKs by statins or other selective inhibitors leads to the upregulation and activation of endothelial nitric oxide synthase (eNOS) and reduction of vascular inflammation and atherosclerosis.
• Source: Sf9 insect cells
• Species: Mouse
• Tag: His
• Bio-activity: The specific activity of ROCK2 was determined to be 209 nmol/min/mg as per actity assay protocol.
• Molecular Mass: Observed MW: ~64 kDa.
  Calculated MW: ~63 kDa.
• Protein length: 5-554
• Purity: The purity of ROCK2 was determined to be > 85 % by densitometry.
• Stability: One year at -70 centigrade from date of shipment.
• Storage: Store product at -70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.1 μg/μL
• Storage Buffer: Recombinant protein stored in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.25mM DTT, 25% glycerol.
• Shipping: Dry ice

Gene Information

• Gene Name: Rock2 Rho-associated coiled-coil containing protein kinase 2 [ Mus musculus ]
• Official Symbol: ROCK2
• Synonyms: ROCK2; Rho-associated coiled-coil containing protein kinase 2; rho-associated protein kinase 2; p164 ROCK-2; Rho-associated coiled-coil forming kinage 2; rho-associated, coiled-coil-containing protein kinase 2; rho-associated, coiled-coil-containing protein kinase II; Rock2m; Rock-II; ROKalpha; mKIAA0619; Rho-kinase; B230113H15Rik
• Gene ID: 19878
• mRNA Refseq: NM_009072
• Protein Refseq: NP_033098