Active Recombinant Human RAN protein, His-tagged

• Cat.No.: RAN-17H
• Product Overview: Recombinant Human RAN fused with His tag at N-terminal was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: Ran (TC4), a small GTP binding protein, is the heart of the nuclear transport process. It is involved in the directionality of the process that is regulated by GTP hydrolysis. Ran shuttles between the nucleus and the cytoplasm. In the nucleus, it exists in Ran-GTP form, which is maintained by the Ran-Guanine nucleotide exchange protein, RCC1, a nucleus specific, chromatin bound protein. Ran-GTP forms complexes with transport proteins that shuttle from the nucleus to the cytoplasm i.e. improtin β, CRM1/exportin1. In the cytoplasm, Ran intrinsic GTPase activity is stimulated by Ran-GAP causing the hydrolysis of GTP to GDP and disassembly of the resulted Ran-GDP from the transport protein. Ran-GDP reenters the nucleus by active transport as NTF2/Ran-GDP complex.
• Form: Solution in 20 mM HEPES-KOH, 110 mM potassium acetate, 2 mM magnesium acetate, 0.5 mM EGTA, 2 mM DTT, and 5% glycerol, pH 7.3.
• Bio-activity: Binds GDP at more than 0.1 molar ratio.
• Purity: >90% (SDS-PAGE)
• Applications: Ran, N-terminal histidine tagged, is a 26.6 kDa protein. It appears on SDS-PAGE as a 31 kDa protein in low ionic strength buffer. In high ionic strength buffers, it appears as a ladder of bands probably representing oligomerization. Ran was purified in the presence of GDP.
• Storage: Store product at -70 centigrade.

Gene Information

• Gene Name: RAN RAN, member RAS oncogene family [ Homo sapiens ]
• Official Symbol: RAN
• Synonyms: RAN; RAN, member RAS oncogene family; GTP-binding nuclear protein Ran; ARA24; Gsp1; TC4; RanGTPase; GTPase Ran; OK/SW-cl.81; ras-like protein TC4; member RAS oncogene family; ras-related nuclear protein; guanosine triphosphatase Ran; androgen receptor-associated protein 24
• Gene ID: 5901
• mRNA Refseq: NM_006325
• Protein Refseq: NP_006316
• MIM: 601179
• UniProt ID: P62826