Active Recombinant Human PTPN1 Protein, Untagged

• Cat.No.: PTPN1-081H
• Product Overview: Active human recombinant PTP1B protein (1-321) without tag was purified from E. coli.

Specification

• Description: The protein encoded by this gene is the founding member of the protein tyrosine phosphatase (PTP) family, which was isolated and identified based on its enzymatic activity and amino acid sequence. PTPs catalyze the hydrolysis of the phosphate monoesters specifically on tyrosine residues. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP has been shown to act as a negative regulator of insulin signaling by dephosphorylating the phosphotryosine residues of insulin receptor kinase. This PTP was also reported to dephosphorylate epidermal growth factor receptor kinase, as well as JAK2 and TYK2 kinases, which implicated the role of this PTP in cell growth control, and cell response to interferon stimulation. Two transcript variants encoding different isoforms have been found for this gene.
• Source: E. coli
• Species: Human
• Molecular Mass: 37.3 kDa
• Purity: ≥95% as determined by SDS-PAGE
• Unit Definition: One unit of enzyme produces 1 μmol of p-nitrophenol/min at 25 centigrade in 100 mM sodium citrate, pH 6.0, containing 10 mM p-nitrophenyl phosphate.
• Stability: ≥ 1 year
• Storage: At -80 centigrade.
• Storage Buffer: 100 mM Tris, pH 7.2, with 100 mM sodium chloride, 1 mM EDTA, and 25% glycerol
• Tag: Tag Free
• Protein length: 1-321 a.a.

Gene Information

• Gene Name: PTPN1 protein tyrosine phosphatase non-receptor type 1 [ Homo sapiens (human) ]
• Official Symbol: PTPN1
• Synonyms: PTPN1; protein tyrosine phosphatase non-receptor type 1; PTP1B; tyrosine-protein phosphatase non-receptor type 1; protein tyrosine phosphatase, placental; protein-tyrosine phosphatase 1B; EC 3.1.3.48
• Gene ID: 5770
• mRNA Refseq: NM_002827
• Protein Refseq: NP_002818
• MIM: 176885
• UniProt ID: P18031