Active Recombinant Human PSIP1, 1-333aa

• Cat.No.: PSIP1-1075H

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Description: The human p52 protein is a non-TAF transcription coactivator that mediates activator-dependent transcription by RNA Polymerase II. The function of p52 is through interactions with transcriptional activators and the basal transcription machinery. In addition, p52 could also interact with several cellular proteins including the transcription coactivator PC4, the essential splicing factor ASF/SF2 and the nuclear protein nucleolin. Recombinant p52 protein (wild type, 333 amino acids) is isolated from an E.coli strain that carries the coding sequence of human p52 under the control of T7 promoter and purified by the combination of an affinity column and FPLC chromatography. Recombinant p52 has been utilized for in vitro function studies, including transcription, splicing, protein-DNA/RNA and protein-protein interactions. Protein is greater than 95% homogeneous and contains no detectable protease, DNase, and RNase activity.
• Form: Liquid. Supplied in 20mM Tris-HCl pH7.9, 20% glycerol, 100mM KCl, 0.2mM EDTA and 1mM DTT.
• Activity: 1ng are sufficient for an in vitro transcription assay and 100ng are sufficient for a protein-protein interaction assay.
• Purity: >95% by SDS-PAGE.
• Usage: For in vitro use only.
• Storage: Quality guaranteed for 12 months. Store at -80°C. Avoid freeze / thaw cycles.

Gene Information

• Gene Name: PSIP1 PC4 and SFRS1 interacting protein 1 [ Homo sapiens ]
• Synonyms: PSIP1; PC4 and SFRS1 interacting protein 1; p52; p75; PAIP; DFS70; LEDGF; PSIP2; MGC74712; transcriptional coactivator p52/p75; PC4 and SFRS1 interacting protein 2; OTTHUMP00000022753; OTTHUMP00000022754; OTTHUMP00000022756; CLL-associated antigen KW-7; Transcriptional coactivator p75/p52; Dense fine speckles 70 kDa protein; Lens epithelium-derived growth factor
• Gene ID: 11168
• mRNA Refseq: NM_001128217
• Protein Refseq: NP_001121689
• MIM: 603620
• UniProt ID: O75475
• Chromosome Location: 9p22.2
• Pathway: HIV Infection
• Function: DNA binding