Active Recombinant Human PSG6 protein, His-tagged

• Cat.No.: PSG6-201H
• Product Overview: Recombinant Human PSG6(Gln35-His424) fused with His tag at C-terminal was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 35-424 a.a.
• Description: Pregnancy-specific glycoprotein 6 (PSG-6), also known as PSG10 and PSGGB is a secreted glycoprotein of the PSG family within the CEA (carcinoembryonic antigen) superfamily. Mature human PSG-6 is a 401 amino acid (aa) protein that consists of four tandem immunoglobulin-like domains followed by a 30 aa C-terminal tail. The N-terminal Ig-like domain contains an Arg-Gly-Asp (RGD) sequence which is a consensus integrin-binding motif found in many adhesion proteins. Alternative splicing generates an additional isoform with a substitution in the C-terminal tail. PSG-6 is predominantly expressed in placenta and more weakly in granulocytes. It induces monocyte production of the cytokines IL-6, IL-10, and TGF-beta 1.
• Predicted N Terminal: No results obtained. Gln35 inferred from enzymatic pyroglutamate treatment revealing Val36
• Form: Lyophilized from a 0.2 μm filtered solution in PBS.
• Bio-activity: Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells. The ED50 for this effect is 0.9-4.5 μg/mL.
• Molecular Mass: Predicted Molecular Mass: 45 kDa
  SDS-PAGE: 62-73 kDa, reducing conditions
• Endotoxin: <0.1 EU per 1 μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Reconstitution: Reconstitute at 500 μg/mL in PBS.

Gene Information

• Gene Name: PSG6 pregnancy specific beta-1-glycoprotein 6 [ Homo sapiens ]
• Official Symbol: PSG6
• Synonyms: PSG6; pregnancy specific beta-1-glycoprotein 6; pregnancy-specific beta-1-glycoprotein 6; PS-beta-G-6; PS-beta-G-10; PS-beta-G-12; pregnancy-specific beta-1-glycoprotein 10; pregnancy-specific beta-1-glycoprotein 12; PSG10; PSBG-6; PSBG-10; PSBG-12
• Gene ID: 5675
• mRNA Refseq: NM_001031850
• Protein Refseq: NP_001027020
• MIM: 176395
• UniProt ID: Q00889