Active Recombinant Human protein kinase C, Alpha, His-tagged

• Cat.No.: PRKCA-58H
• Product Overview: Recombinant Human Protein Kinase C alpha produced inSf9is a glycosylated, polypeptide chain containing amino acids 2-672 and having a molecular mass of 80 KD. This protein is the full-length form of the protein with an amino terminal poly His-tag. PKC- is purified by proprietary chromatographic techniques.

Specification

• Cat. No.: PRKCA-58H
• Description: Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells.
• Source: Sf9 insect cells.
• Unit Definition: TheUnit Definition is 7200-9000 units/mg. One unit of PKC-activity transfers 1 nmole of phosphate to a PKC substrate peptide (myelin basic protein 4-14) per minute at 30°C. Recombinant human active PKC-also phosphorylates full length myelin basic protein, histone, MARKS, and GSK-3. Activity requires incubation with lipid activators, and may vary depending on the substrate and reaction conditions.
• Purity: Greater than 95% as determined by SDS-PAGE.
• Formulation: PKC- is supplied at a of 0.3mg/ml in 50mM Tris, pH 7.4, 0.1M NaCl, 20% glycerol, 1mM DTT, 0.1mM EDTA, 0.2mM PMSF and 0.03% Brij-35.
• Storage: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles.
• Tag: His
• Protein length: 2-672 a.a.

Gene Information

• Gene Name: PRKCA protein kinase C, alpha [ Homo sapiens ]
• Synonyms: PRKCA; protein kinase C, alpha; AAG6; PKCA; PRKACA; MGC129900; MGC129901; PKC-alpha;EC2.7.11. 13;Protein kinase C alpha type; aging-associated gene 6; protein kinase C alpha type
• Gene ID: 5578
• mRNA Refseq: NM_002737
• Protein Refseq: NP_002728
• UniProt ID: P17252
• MIM: 176960
• Chromosome Location: 17q22-q24
• Pathway: Calcium signaling pathway; ErbB signaling pathway; Fc epsilon RI signaling pathway; Fc gamma R-mediated phagocytosis; Focal adhesion; Glioma; GnRH signaling pathway; Leukocyte transendothelial migration; Long-term potentiation; Long-term depression; MAPK signaling pathway; Melanogenesis; Natural killer cell mediated cytotoxicity; Non-small cell lung cancer; Pathogenic Escherichia coli infection - EHEC; Pathways in cancer; Phosphatidylinositol signaling system; Tight junction; VEGF signaling pathway; Vascular smooth muscle contraction; Vibrio cholerae infection; Wnt signaling pathway; Diabetes pathways; Hemostasis; Synaptic Transmission
• Function: ATP binding; calcium ion binding; calcium-dependent protein kinase C activity; nucleotide binding; protein binding; transferase activity; zinc ion binding