Active Recombinant Full Length Human PRKCA Protein, GST-tagged

• Cat.No.: PRKCA-3385HF
• Product Overview: Full-length human PKCα [1-672(end) amino acids of accession number NP_002728.1] was expressed as N-terminal GST-fusion protein (104 kDa) using baculovirus expression system. GST-PKCα was purified by using glutathione sepharose chromatography.

Specification

• Source: Insect (sf21)
• Species: Human
• Tag: GST
• Form: 50 mM Tris-HCl, 150 mM NaCl, 0.2% Brij35, 1 mM DTT, 10% glycerol, pH7.5
• Bio-activity: The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorecence-labeled substrate, Mg(or Mn)/ATP, and Ca/Lipid Activator. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip™3000.
• Protein length: 1-672(end)
• Purity: 96%
• Applications: SDS-PAGE
• Storage: Store at -80C. Avoid repeating freeze-thaws.

Gene Information

• Gene Name: protein kinase C, alpha
• Official Symbol: PRKCA
• Synonyms: PRKCA;AAG6;PKC-alpha;PKCA;PKCI+/-;PKCalpha;PRKACA
• Protein Refseq: NP_002728.1
• UniProt ID: P17252