Recombinant Human Protein Kinase C, Alpha, GST-tagged

• Cat.No.: PRKCA-1087H
• Product Overview: Recombinant full-length human PRKCA alpha was expressed by baculovirus in Sf9 insect cells using a N-terminal GST tag.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: GST
• Description: PRKCA is a member of the protein kinase C (PKC) family of serine-and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PRKCA has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. PRKCA has been assigned to the chromosome region 17q22-q23.2 and has been identified as a fundamental regulator of cardiac contractility and Ca2+ handling in myocytes.
• Applications: Kinase Assay; Western Blot
• Molecular Weight: 103 kDa
• Expression System: Sf9 insect cells using baculovirus
• Form: Recombinant protein stored in 50 mM sodium phosphate, pH 7.5, 150 mM NaCl, 0.1 mM PMSF, 0.25 mM DTT, 25% glycerol, 0.1 mM EGTA, 0.1 mM EDTA.
• Specific Activity: 3333 nmol/min/mg
• Purity: > 90 %
• Concentration: 0.1 ug/ul
• Storage: Store product at -70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Gene Information

• Gene Name: PRKCA protein kinase C, alpha [ Homo sapiens ]
• Official Symbol: PRKCA
• Synonyms: PRKCA; protein kinase C, alpha; AAG6; PKCA; PRKACA; MGC129900; MGC129901; PKC-alpha; EC 2.7.11. 13; Protein kinase C alpha type; aging-associated gene 6; protein kinase C alpha type
• Gene ID: 5578
• mRNA Refseq: NM_002737
• Protein Refseq: NP_002728
• MIM: 176960
• UniProt ID: P17252
• Chromosome Location: 17q22-q24
• Function: ATP binding; enzyme binding; histone kinase activity (H3-T6 specific); metal ion binding; nucleotide binding; protein binding; protein kinase C activity; protein kinase activity; protein serine/threonine kinase activity; zinc ion binding