Active Recombinant Human PRLR protein, Mouse IgG2a Fc-tagged

• Cat.No.: PRLR-0638H
• Product Overview: Recombinant Human PRLR protein(Gln25-Asp234), fused with Fc tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: mIgG2a
• Protein Length: 25-234 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human Prolactin, Mouse IgG2a Fc Tag, low endotoxin at 1 μg/mL (100 μL/well) can bind Human Prolactin R, Fc Tag with a linear range of 2-39 ng/mL. Immobilized Human GH, Tag Free at 2 μg/mL (100 μL/well) can bind Human Prolactin R, Fc Tag with a linear range of 0.4-6 ng/mL.
• Molecular Mass: This protein carries a human IgG1 Fc tag at the C-terminus.The protein has a calculated MW of 51.0 kDa. The protein migrates as 64 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: PRLR prolactin receptor [ Homo sapiens ]
• Official Symbol: PRLR
• Synonyms: PRLR; prolactin receptor; PRL-R; hPRL receptor; secreted prolactin binding protein; hPRLrI
• Gene ID: 5618
• mRNA Refseq: NM_000949
• Protein Refseq: NP_000940
• MIM: 176761
• UniProt ID: P16471