Active Recombinant Human Pim-3, His-tagged

• Cat.No.: Pim3-116H
• Product Overview: Recombinant Human Pim-3 fused with 6­His tag at N­terminal, was expressed in Sf21 insect cells.

Specification

• Species: Human
• Source: Sf21 Cells
• Tag: His
• Description: The protein encoded by this gene belongs to the Ser/Thr protein kinase family, and PIM subfamily. This gene is overexpressed in hematological and epithelial tumors and is associated with MYC coexpression. It plays a role in the regulation of signal transduction cascades, contributing to both cell proliferation and survival, and provides a selective advantage in tumorigenesis.
• Form: 1.05mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 5210U/mg, where one unit of Pim-3 activity is defined as 1nmol phosphate incorporated into 300µM (RSRHSSYPAGT) per minute at 30°C with a final ATP concentration of 100µM.
• Molecular Mass: 39.6 kDa
• Storage: Stable for 6 months at -70°C from date of shipment. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: PIM3 pim-3 oncogene [ Homo sapiens (human) ]
• Official Symbol: PIM3
• Synonyms: PIM3; pim-3; pim-3 oncogene; serine/threonine-protein kinase pim-3; serine/threonine kinase Pim-3; EC 2.7.11.1
• Gene ID: 415116
• mRNA Refseq: NM_001001852
• Protein Refseq: NP_001001852
• MIM: 610580
• UniProt ID: Q86V86
• Chromosome Location: 22q13
• Function: ATP binding; protein binding; protein serine/threonine kinase activity