Active Recombinant Human PI4K2A protein, GST-tagged

• Cat.No.: PI4K2A-159H
• Product Overview: Recombinant Human PI4K2A fused with an N-terminal GST tag was expressed in Insect cells.

Specification

• Species: Human
• Source: Insect Cells
• Tag: GST
• Description: PI4K2A or phosphatidylinositol 4-kinase type 2 alpha is involved in the formation of phosphatidylinositol-polyphosphates (PtdInsPs) which are centrally involved in many biologic processes ranging from cell growth and organization of the actin cytoskeleton to endo- and exocytosis. PI4K2A phosphorylates PtdIns at the D-4 position which is an essential step in the biosynthesis of PtdInsPs. PI4K2A is a novel regulator of tumor growth by its action on angiogenesis and HIF-1alpha regulation. PI4K2A generates PtdIns4P-rich domains within the Golgi which regulate targeting of clathrin adaptor AP-1 complexes. PI4K2A is also required for Wnt to regulate LRP6 phosphorylation.
• Form: 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Bio-activity: 1500 nmol/min/mg
• Molecular Mass: ~79 kDa
• Purity: >95%
• Applications: Kinase Assay
• Stability: 1 year at -70 centigrade from the date of shipment
• Storage: Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.1μg/μl

Gene Information

• Gene Name: PI4K2A phosphatidylinositol 4-kinase type 2 alpha [ Homo sapiens ]
• Official Symbol: PI4K2A
• Synonyms: PI4K2A; phosphatidylinositol 4-kinase type 2 alpha; phosphatidylinositol 4-kinase type 2-alpha; DKFZP761G1923; PI4KII; PIK42A; phosphatidylinositol 4-kinase type II-alpha; phosphatidylinositol 4-kinase type II (PI4KII); RP11-548K23.6; DKFZp761G1923
• Gene ID: 55361
• mRNA Refseq: NM_018425
• Protein Refseq: NP_060895
• MIM: 609763
• UniProt ID: Q9BTU6
• Chromosome Location: 10q24
• Pathway: 3-phosphoinositide biosynthesis, organism-specific biosystem; 3-phosphoinositide biosynthesis, conserved biosystem; Canonical Wnt signaling pathway, organism-specific biosystem; D-myo-inositol (1,4,5)-trisphosphate biosynthesis, organism-specific biosystem; D-myo-inositol (1,4,5)-trisphosphate biosynthesis, conserved biosystem; Inositol phosphate metabolism, organism-specific biosystem; Inositol phosphate metabolism, conserved biosystem
• Function: 1-phosphatidylinositol 4-kinase activity; ATP binding; kinase activity; magnesium ion binding; nucleotide binding; transferase activity