Active Recombinant Human PCSK9, His-tagged, Biotinylated

• Cat.No.: PCSK9-186H
• Product Overview: Biotinylated Human PCSK9 Protein,Superior Sensitivity (Biotinylated PCSK9) Gln 31 - Gln 692 (AAI66619), was chemically labeled with specially optimized long spacer biotin reagent based on rhPCSK9 Protein, which was produced in human 293 cells (HEK293).

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 31-692 a.a.
• Description: Proprotein convertase subtilisin/kexin type 9 (PCSK9), is an enzyme which in humans is encoded by the PCSK9 gene. This gene encodes a proprotein convertase belonging to the proteinase K subfamily of the secretory subtilase family. This protein plays a major regulatory role in cholesterol homeostasis. PCSK9 binds to the epidermal growth factor-like repeat A (EGF-A) domain of the low-density lipoprotein receptor (LDLR), inducing LDLR degradation. PCSK9 may also have a role in the differentiation of cortical neurons. Mutations in this gene have been associated with a rare form of autosomal dominant familial hypercholesterolemia (HCHOLA3).The primary amine of rhPCSK9 was chemically labeled by specially optimized long spacer biotin reagent without decreasing its bioactivity. Biotinylated PCSK9 protein, Superior Sensitivity has been used successfully for easy detection in ELISA, dot blot or Western blot, immunohistochemistry applications using streptavidin or avidin-conjugated probes. Biotin reagent with optimized long spacer provides improved biotin exposure and less steric hindrance,thus giving superior sensitivity and response when detection with streptavidin. Biotin and other reactive related chemicals have been removed thoroughly by desalting after reaction termination.
• Predicted N Terminal: Gln 31
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.
• Bio-activity: Measured by its biotin incorporate rate in a ELISA assay. Immobilized Biotinylated rhPCSK9 at diffrent concentration (100 μL/well), when the concentration is 0.5-50 ng/mL, Biotinylated rhPCSK9 can be detected by HRP*-Labeled Streptavidin with a linear ran
• Molecular Mass: Biotinylated rhPCSK9 is fused with polyhistidine tag at the C-terminus, and has a calculated MW of 75.1 kDa. The predicted N-terminus is Gln 31. DTT-reduced Protein migrates as 18 kDa and 67 kDa in SDS-PAGE due to glycosylation and proteolytic digestion.
• Endotoxin: Less than 1.0 EU per μg of the Biotinylated rh PCSK9 by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Applications: Biotin Labeled Protein
• Storage: Avoid repeated freeze-thaw cycles.No activity loss was observed after storage at:In lyophilized state for 1 year (4oC); After reconstitution under sterile conditions for 3 months (-70oC).

Gene Information

• Gene Name: PCSK9 proprotein convertase subtilisin/kexin type 9 [ Homo sapiens ]
• Official Symbol: PCSK9
• Synonyms: PCSK9; proprotein convertase subtilisin/kexin type 9; HCHOLA3, hypercholesterolemia, autosomal dominant 3; FH3; NARC 1; NARC1; NARC-1; HCHOLA3
• Gene ID: 353175
• MIM: 607786
• UniProt ID: Q8NBP7
• Chromosome Location: 1p34.1-p32