Active Recombinant Human NTF3 Protein

• Cat.No.: NT3-01H
• Product Overview: Recombinant Human NTF3 Protein, Tyr139-Thr257 (K196R), without tag was expresssed in Spodoptera frugiperda, Sf 21 (baculovirus).

Specification

• Species: Human
• Source: Insect Cells
• Protein Length: 139-257
• Description: The protein encoded by this gene is a member of the neurotrophin family, that controls survival and differentiation of mammalian neurons. This protein is closely related to both nerve growth factor and brain-derived neurotrophic factor. It may be involved in the maintenance of the adult nervous system, and may affect development of neurons in the embryo when it is expressed in human placenta. NTF3-deficient mice generated by gene targeting display severe movement defects of the limbs. The mature peptide of this protein is identical in all mammals examined including human, pig, rat and mouse.
• Bio-activity: Measured in a cell proliferation assay using BaF mouse pro-B cells transfected with TrkB. The ED50 for this effect is 4-40 ng/mL.
  The specific activity of Recombinant Human NT-3 is approximately 2082 U/μg, which is calibrated against recombinant human NT-3 Standard.
• Molecular Mass: 13.7 kDa
• N-terminal Sequence Analysis: Tyr139
• Endotoxin: < 0.10 EU/μg of the protein by the LAL method.
• Purity: >97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Reconstitution: Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
• Shipping: At ambient temperature.

Gene Information

• Gene Name: NTF3 neurotrophin 3 [ Homo sapiens (human) ]
• Official Symbol: NTF3
• Synonyms: NTF3; neurotrophin 3; NT3; HDNF; NGF2; NT-3; NGF-2; neurotrophin-3; nerve growth factor 2; neurotrophic factor
• Gene ID: 4908
• mRNA Refseq: NM_002527
• Protein Refseq: NP_002518
• MIM: 162660
• UniProt ID: P20783