Active Recombinant Human NT3 protein(Tyr139-Thr257)

• Cat.No.: NT3-205H
• Product Overview: Recombinant Human NT3 (P20783-1) (Tyr139-Thr257) was expressed and purified in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Protein Length: Tyr139-Thr257
• Form: Lyophilized from sterile PBS, pH 5.2. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized human NT3 at 2 μg/ml (100 μl/well) can bind human TrkB-Fch. The EC50 of human TrkB-Fch is 120-350 ng/mL.
• Molecular Mass: The recombinant human NT3 consists of 120 amino acids and has a predicted molecular mass of 13.8 kDa. The apparent molecular mass of it is approximately 16 kDa in SDS-PAGE under reducing conditions.
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: NT3 3-nucleotidase [ Homo sapiens ]
• Official Symbol: NT3
• Synonyms: NT3; 3-nucleotidase
• Gene ID: 4877