| Species : |
Human |
| Source : |
E.coli |
| Tag : |
GST |
| Protein Length : |
1-53 aa |
| Description : |
Neuregulin-4 (Nrg4) belongs to a small family of EGF-like (EGFL) domain-containing proteins that are synthesized as transmembrane precursors and undergo proteolytic cleavage. The EGF-like domain (aa 5-46) of Nrg4 (aa 1-53) directly binds to the receptors ErbB3 and 4. Nrg4 is a cold induced adipokine, highly expressed in adipose tissues and enriched in brown fat. It is increased during brown adipocyte differentiation and reduced in rodent and human obesity. It promotes neurite outgrowth and protects against diet-induced insulin resistance and hepatic steatosis through attenuating hepatic lipogenic signaling. This hepatic effect of Nrg4 is mediated by ErbB3 and ErbB4 signaling that negatively regulates de novo lipogenesis mediated by LXR and SREBP1c. This effect of Nrg4 on fatty liver and insulin resistance could lead to the development of Nrg4 as an effective therapeutic biological for the treatment of NAFLD and type 2 diabetes. |
| Bio-activity : |
Binds to the human receptor tyrosine-protein kinase ErbB4. |
| Molecular Mass : |
~32 kDa (SDS-PAGE) |
| Endotoxin : |
< 0.01 EU/μg purified protein (LAL test). |
| Purity : |
≥ 95% estimated by SDS-PAGE |
| Stability : |
Stable for at least 6 months after receipt when stored at -20 centigrade.
Working aliquots are stable for up to 3 months when stored at -20 centigrade. |
| Storage : |
Short Term Storage: +4 centigrade
Long Term Storage: -20 centigrade |
| Storage Buffer : |
Lyophilized. Contains PBS. |
| Concentration : |
0.1 mg/mL after reconstitution. |
| Shipping : |
BLUE ICE |
| Handling : |
After reconstitution, prepare aliquots and store at -20 centigrade. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1% BSA should be used for further dilutions. |
| Reconstitution : |
Reconstitute with 100 μL sterile water. |
