Active Recombinant Human NMNAT1 protein, His-tagged

Description :	NMNAT1, also known as NMNAT or PNAT1, is a central enzyme in NAD biosynthesis, catalyzing the condensation of nicotinamide mononucleotide (NMN) or nicotinic acid mononucleotide (NaMN) with the AMP moiety of ATP to form NAD or NaAD. It is widely expressed with high levels in skeletal muscle, heart, liver and kidney. This protein appears to have the ability to protect against axonal degeneration following mechanical or toxic insults.
Source :	E. coli
Species :	Human
Tag :	His
Form :	Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT. 1mM EDTA
Bio-activity :	Specific activity is > 7,000 pmol/min/ug, and was obtained by measuring the beta-NAD from nicotinamide mononucleotide and ATP per minute at pH 8.0 at 37C.
Molecular Mass :	36.0 kDa (315aa) confirmed by MALDI-TOF
AA Sequence :	MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMENS EKTEVVLLAC GSFNPITNMH LRLFELAKDY MNGTGRYTVV KGIISPVGDAYKKKGLIPAY HRVIMAELAT KNSKWVEVDT WESLQKEWKE TLKVLRHHQE KLEASDCDHQ QNSPTLERPG RKRKWTETQD SSQKKSLEPKTKAVPKVKLL CGADLLESFA VPNLWKSEDI TQIVANYGLI CVTRAGNDAQ KFIYESDVLW KHRSNIHVVN EWIANDISST KIRRALRRGQSIRYLVPDLV QEYIEKHNLY SSESEDRNAG VILAPLQRNT AEAK
Purity :	> 95% by SDS - PAGE
Storage :	Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
Concentration :	1 mg/ml (determined by Bradford assay)
Protein length :	115-270 a.a.

Gene Name	NMNAT1 nicotinamide nucleotide adenylyltransferase 1 [ Homo sapiens ]
Official Symbol	NMNAT1
Synonyms	NMNAT1; nicotinamide nucleotide adenylyltransferase 1; nicotinamide nucleotide adenylyltransferase; nicotinamide mononucleotide adenylyltransferase 1; NMNAT; PNAT1; NMN adenylyltransferase 1; NaMN adenylyltransferase 1; pyridine nucleotide adenylyltransferase 1; nicotinate-nucleotide adenylyltransferase 1;
Gene ID	64802
mRNA Refseq	NM_022787
Protein Refseq	NP_073624
MIM	608700
UniProt ID	Q9HAN9
Chromosome Location	1p36.22
Pathway	Metabolic pathways, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of vitamins and cofactors, organism-specific biosystem; Metabolism of water-soluble vitamins and cofactors, organism-specific biosystem; NAD biosynthesis II (from tryptophan), organism-specific biosystem; NAD biosynthesis III, organism-specific biosystem; NAD biosynthesis from 2-amino-3-carboxymuconate semialdehyde, organism-specific biosystem;
Function	ATP binding; nicotinamide-nucleotide adenylyltransferase activity; nicotinamide-nucleotide adenylyltransferase activity; nicotinate-nucleotide adenylyltransferase activity; nucleotide binding; nucleotidyltransferase activity; protein binding; transferase activity;