Recombinant Human NMNAT1, His-tagged

• Cat.No.: NMNAT1-2766H
• Product Overview: Recombinant human NMNAT1 protein, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography techniques.MW = 36.0 kDa (315aa).

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: NMNAT1, also known as NMNAT or PNAT1, is a central enzyme in NAD biosynthesis, catalyzing the condensation of nicotinamide mononucleotide (NMN) or nicotinic acid mononucleotide (NaMN) with the AMP moiety of ATP to form NAD or NaAD. It is widely expressed with high levels in skeletal muscle, heart, liver and kidney. This protein appears to have the ability to protect against axonal degeneration following mechanical or toxic insults.
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol 0.1M NaCl, 1mM DTT, 1mM EDTA.
• Purity: > 95% by SDS - PAGE.
• Concentration: 1.0 mg/ml (determined by Bradford assay).
• Sequences Of Amino Acids: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMENS EKTEVVLLAC GS FNPITNMH LRLFELAKDY MNGTGRYTVV KGIISPVGDAYKKKGLIPAY HR VIM AEL AT KNSKWVEVDT WESLQKEWKE TLKVLRHHQE KLEASDCDHQ QN SPT LERPG RKRKWTETQD SSQKKSLEPK TKAVPKVKLL CGADLLESFA VPNLWKSEDI TQIVANYGLI CVTRAGNDAQ KFIYESDVLW KHRSNIHVVN EWIANDISST KIRRALRRGQSIRYLVPDLV QEYIEKHNLY SSESEDRNAG VILAPLQRNT AEAKT.
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Pathways: Metabolic pathways; Nicotinate and nicotinamide metabolism; Metabolism of vitamins and cofactors

Gene Information

• Gene Name: NMNAT1 nicotinamide nucleotide adenylyltransferase 1 [ Homo sapiens ]
• Synonyms: nicotinamide nucleotide adenylyltransferase 1; NMNAT; PNAT1; NMNAT1; nicotinamide mononucleotide adenylyltransferase 1; OTTHUMP00000001732; NMN adenylyltransferase 1; NaMN adenylyltransferase 1; pyridine nucleotide adenylyltransferase 1; nicotinate-nucleotide adenylyltransferase 1; Nicotinate-nucleotide adenylyltransferase 1; nicotinamide nucleotide adenylyltransferase; EC 2.7.7.1,EC 2.7.7.18
• Gene ID: 64802
• mRNA Refseq: NM_022787
• Protein Refseq: NP_073624
• MIM: 608700
• UniProt ID: Q9HAN9
• Chromosome Location: 1p36.22
• Function: ATP binding; nicotinamide-nucleotide adenylyltransferase activity; nicotinate-nucleotide adenylyltransferase activity; nucleotide binding; nucleotidyltransferase activity; protein binding; transferase activity