Active Recombinant Human NLK protein, His&GST-tagged

• Cat.No.: NLK-7318H
• Product Overview: Recombinant Human NLK protein(Q9UBE8)(Val121-Glu527), fused with His tag and GST tag, was expressed in Insect Cells.

Specification

• Species: Human
• Source: Insect Cells
• Tag: GST&His
• Protein Length: 121-527 a.a.
• Form: Supplied as sterile 20mM Tris, 500mM NaCl, pH 8.0, 10% glyPlease contact us for any concerns or special requirements.Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: The specific activity was determined to be 3 nmol/min/mg using MBP as substrate.
• Molecular Mass: The recombinant human NLK /GST chimera consists of 644 amino acids and has a calculated molecular mass of 74.1 kDa. The recombinant protein migrates as an approximately 73 kDa band in SDS-PAGE under reducing conditions.
• Proteinlength: Val121-Glu527
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 91 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: NLK nemo-like kinase [ Homo sapiens ]
• Official Symbol: NLK
• Synonyms: NLK; nemo-like kinase; nemo like kinase; serine/threonine-protein kinase NLK; FLJ21033; DKFZp761G1211
• Gene ID: 51701
• mRNA Refseq: NM_016231
• Protein Refseq: NP_057315
• MIM: 609476
• UniProt ID: Q9UBE8