Active Recombinant Human NLK, GST-tagged

• Cat.No.: NLK-1066H
• Product Overview: Recombinant full-length human NLK was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Description: NLK (also known as nemo-like kinase) is a member of the serine/threonine protein kinase that is encoded by the NLK gene. NLK plays a role in cell fate determination and is required for the differentiation of bone marrow stromal cells. NLK also suppresses a wide range of transcription factors such as CREB by phosphorylation of the co-activator CREBBP. NLK expression increases the number of apoptotic cells but does not induce obvious cell cycle arrest. NLK suppresses Notch signalling by interfering with formation of the Notch active transcriptional complex.
• Source: Sf9 insect cells
• Species: Human
• Tag: GST
• Form: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Bio-activity: 2.4 nmol/min/mg
• Molecular Mass: ~84 kDa
• Protein length: Full Length
• Purity: >85%
• Applications: Western Blot
• Stability: 1 yr at -70oC from date of shipment.
• Storage: Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.1 ug/ul

Gene Information

• Gene Name: NLK nemo-like kinase [ Homo sapiens ]
• Official Symbol: NLK
• Synonyms: NLK; nemo-like kinase; serine/threonine-protein kinase NLK; NP_057315.3; EC 2.7.11.24
• Gene ID: 51701
• mRNA Refseq: NM_016231
• Protein Refseq: NP_057315
• MIM: 609476
• UniProt ID: Q9UBE8
• Chromosome Location: 17q11.2
• Pathway: Adherens junction; C-MYB transcription factor network; IL-6 Signaling Pathway; MAPK signaling pathway
• Function: ATP binding; MAP kinase activity; SH2 domain binding; magnesium ion binding