Active Recombinant Human Neurocan, His-tagged
Cat.No. : | NCAN-5691H |
Product Overview : | Recombinant Human NCANprotein was expressed in CHO with a C-terminal 6-His tag. Glu23-Cys1321. |
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Species : | Human |
Source : | CHO |
Tag : | His |
Protein Length : | 23-1321 a.a. |
Description : | NCAN, also known asCSPG3 and 1D1, is a secreted chondroitin sulfate proteoglycan that isprimarily expressed in the central nervous system. Human NCAN contains oneIg-like V-type domain, two Link domains, two EGF-like domains, one C-typelectin like domain, and one Sushi domain. It is an approximately 300 kDamolecule of which 66 kDa is chondroitin sulfate and 60 kDa is Nand O-linked glycosylation.Mature human NCAN shares 66% aa sequence identity with mature mouse and ratNCAN. |
Form : | Lyophilized from a0.2 μm filtered solution in PBS. |
N-terminalSequence : | Glu23 |
Molecular Weight : | 141.5kDa |
Activity : | Measuredby the ability of the immobilized protein to enhance the adhesion of H4 humanneuroglioma cells. When 5 x 104 cells per well are added to recombinanthuman NCAN coated plates, cell adhesion is enhanced in a dose dependentmanner. The ED50 for this effect is typically 50-200 ng/mL. |
Endotoxin Level : | <1.0 EUper 1 μg of the protein by the LAL method. |
SDS-PAGE : | 140-290kDa, reducing conditions. |
Purity : | >90%,by SDS-PAGE under reducing conditions and visualized by silver stain. |
Reconstitution : | 100μg/mL in PBS |
Storage : | Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 12 months fromdate of receipt, -20 to -70°C as supplied. 1 month, 2 to 8°C under sterileconditions after reconstitution. 3 months, -20 to -70°C under sterileconditions after reconstitution. |
Gene Name | NCAN neurocan [ Homo sapiens] |
Official Symbol | NCAN |
Synonyms | NCAN; neurocan; CSPG3;FLJ44681; neurocan core protein; neurocan proteoglycan; chondroitin sulfateproteoglycan 3 (neurocan); NEUR; Chondroitin sulfate proteoglycan 3 |
Gene ID | 1463 |
mRNA Refseq | NM_004386 |
Protein Refseq | NP_004377 |
MIM | 600826 |
UniProt ID | O14594 |
Chromosome Location | 19p12 |
Pathway | Axon guidance; Developmental Biology; L1CAMinteractions; NCAM signaling for neurite out-growth; NCAM1 interactions;Syndecan-3-mediated signaling events |
Function | binding; calcium ion binding; hyaluronicacid binding; sugar binding |
◆ Recombinant Proteins | ||
NCAN-5929M | Recombinant Mouse NCAN Protein, His (Fc)-Avi-tagged | +Inquiry |
NCAN-490H | Recombinant Human NCAN | +Inquiry |
Ncan-376R | Recombinant Rat Ncan Protein, His-tagged | +Inquiry |
NCAN-2014H | Recombinant Human NCAN Protein, GST-tagged | +Inquiry |
NCAN-374H | Recombinant Human NCAN Protein, His-tagged | +Inquiry |
Not For Human Consumption!
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