Active Recombinant Human NEK1 Protein, GST-tagged

• Cat.No.: NEK1-2993H
• Product Overview: Recombinant human protein, catalytic domain (amino acids 1-505), GST-tagged, expressed in insect cells.

Specification

• Species: Human
• Source: Insect Cells
• Tag: GST
• Protein Length: 1-505
• Description: The protein encoded by this gene is a serine/threonine kinase involved in cell cycle regulation. The encoded protein is found in a centrosomal complex with FEZ1, a neuronal protein that plays a role in axonal development. Defects in this gene are a cause of polycystic kidney disease (PKD). Several transcript variants encoding different isoforms have been found for this gene.
• Bio-activity: 590 nmoles of phosphate transferred to myelin basic protein (MBP) per minute per mg of total protein at 30 centigrade. Activity determined at a final protein concentration of 1.67 μg/mL.
• Molecular Mass: 85.3 kDa
• Purity: 85% as determined by a Coomassie® blue stained SDS-PAGE gel.
• Storage: Store at -80 centigrade. At first use, aliquot and store at -80 centigrade to avoid multiple freeze-thaws.
• Concentration: 0.34 mg/mL total protein as measured using the Bradford protein assay with BSA as a standard.
• Storage Buffer: 50 mM Tris (pH 7.5), 150 mM NaCl, 0.5 mM EDTA, 0.02% Triton® X–100, 2 mM DTT and 50% Glycerol.
• Shipping: Dry Ice

Gene Information

• Gene Name: NEK1 NIMA related kinase 1 [ Homo sapiens (human) ]
• Official Symbol: NEK1
• Synonyms: NEK1; NIMA related kinase 1; ALS24; SRPS2; SRTD6; SRPS2A; NY-REN-55; serine/threonine-protein kinase Nek1; NIMA (never in mitosis gene a)-related kinase 1; never in mitosis A-related kinase 1; nimA-related protein kinase 1; protein-serine/threonine kinase; renal carcinoma antigen NY-REN-55; EC 2.7.11.1
• Gene ID: 4750
• mRNA Refseq: NM_012224
• Protein Refseq: NP_036356
• MIM: 604588
• UniProt ID: Q96PY6