Active Recombinant Human NEDD4 protein, His-tagged

• Cat.No.: NEDD4-01H
• Product Overview: Recombinant Human NEDD4 protein, fused to His-tag, was expressed in E. coli.

Specification

• Description: This gene is the founding member of the NEDD4 family of HECT ubiquitin ligases that function in the ubiquitin proteasome system of protein degradation. The encoded protein contains an N-terminal calcium and phospholipid binding C2 domain followed by multiple tryptophan-rich WW domains and, a C-terminal HECT ubiquitin ligase catalytic domain. It plays critical role in the regulation of a number of membrane receptors, endocytic machinery components and the tumor suppressor PTEN.
• Source: E. coli
• Species: Human
• Tag: His
• Form: X mg/ml (X μM) in 50 mM HEPES pH 8.0, 100 mM NaCl, 5 mM DTT, 1 mM EDTA.
• Bio-activity: Recombinant Human NEDD 4 is a Ubiquitin Ligase (E3) that functions downstream of a Ubiquitin-activating (E1) enzyme and a Ubiquitin-conjugating (E2) enzyme to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application.
• Molecular Mass: 88 kDa
• Purity: >95%
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -70 centigrade as supplied.
  3 months, -70 centigrade under sterile conditions after opening.
• Shipping: The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Gene Information

• Gene Name: NEDD4 NEDD4 E3 ubiquitin protein ligase [ Homo sapiens (human) ]
• Official Symbol: NEDD4
• Synonyms: Cell proliferation-inducing gene 53 protein; E3 ubiquitin-protein ligase NEDD4; EC 6.3.2; EC 6.3.2.-; KIAA0093NEDD4-1MGC176705; NEDD4; NEDD-4; Neural precursor cell expressed developmentally down-regulated protein 4; neural precursor cell expressed, developmentally down-regulated 4; receptor-potentiating factor 1; RPF1
• Gene ID: 4734
• mRNA Refseq: NM_006154
• Protein Refseq: NP_006145
• MIM: 602278
• UniProt ID: P46934