Recombinant Human NEDD8 Activating Enzyme E1 Subunit 1, His-tagged

• Cat.No.: NAE1-1570H
• Product Overview: Recombinant human NAE1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: NAE1 (NEDD8-activating enzyme E1 regulatory subunit) belongs to the ubiquitin-activating E1 family. This protein binds to the beta-amyloid precursor protein. Beta-amyloid precursor protein is a cell surface protein with signal-transducing properties, and it is thought to play a role in the pathogenesis of Alzheimer"s disease. NAE1 participates in a novel ubiquitinylation-related pathway involving the ubiquitin-like molecule NEDD8. Also, this protein is required for cell cycle progression through the S/M checkpoint.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 10% glycerol, 200mM NaCl.
• Molecular Weight: 62.7 kDa (557aa)
• Purity: >90% by SDS-PAGE
• Concentration: 0.5 mg/ml (determined by Bradford assay)
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MGSMAQLGKL LKEQKYDRQL RLWGDHGQEA LESAHVCLIN ATATGTEILK NLVLPGIGSF TIIDGNQVSG EDAGNNFFLQ RSSIGKNRAE AAMEFLQELN SDVSGSFVEE SPENLLDNDP SFFCRFTVVV ATQLPESTSL RLADVLWNSQ IPLLICRTYG LVGYMRIIIK EHPVIESHPD NALEDLRLDK PFPELREHFQ SYDLDHMEKK DHSHTPWIVI IAKYLAQWYS ETNGRIPKTY KEKEDFRDLI RQGILKNENG APEDEENFEE AIKNVNTALN TTQIPSSIED IFNDDRCINI TKQTPSFWIL ARALKEFVAK EGQGNLPVRG TIPDMIADSG KYIKLQNVYR EKAKKDAAAV GNHVAKLLQS IGQAPESISE KELKLLCSNS AFLRVVRCRS LAEEYGLDTI NKDEIISSMD NPDNEIVLYL MLRAVDRFHK QQGRYPGVSN YQVEEDIGKL KSCLTGFLQE YGLSVMVKDD YVHEFCRYGA AEPHTIAAFL GGAAAQEVIK IITKQFVIFN NTYIYSGMSQ TSATFQL
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Pathways: Alzheimer"s disease

Gene Information

• Gene Name: NAE1 NEDD8 activating enzyme E1 subunit 1 [ Homo sapiens ]
• Official Symbol: NAE1
• Synonyms: NAE1; NEDD8 activating enzyme E1 subunit 1; HPP1; ula-1; APPBP1; A-116A10.1; NEDD8-activating enzyme E1 regulatory subunit; APP-BP1; OTTHUMP00000174807; OTTHUMP00000174808; protooncogene protein 1; proto-oncogene protein 1; amyloid protein-binding protein 1; NEDD8-activating enzyme E1 subunit; amyloid beta precursor protein-binding protein 1, 59kD; amyloid beta precursor protein binding protein 1, 59kDa; amyloid beta precursor protein-binding protein 1, 59 kDa; APP-BP1; amyloid beta precursor protein binding protein 1; Amyloid beta precursor protein-binding protein 1, 59 kDa; NEDD8-activating enzyme E1 subunit; Amyloid protein-binding protein 1
• Gene ID: 8883
• mRNA Refseq: NM_001018159
• Protein Refseq: NP_001018169
• MIM: 603385
• UniProt ID: Q13564
• Chromosome Location: 16q22
• Function: Contributes to NEDD8 activating enzyme activity; catalytic activity; protein binding; protein heterodimerization activity