Active Recombinant Human MYLK2, His-tagged

• Cat.No.: MYLK2-1435H
• Product Overview: Recombinant full-length human MYLK2 was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: His
• Protein Length: Full length
• Description: MYLK2 is a member of the myosin light chain kinase family and is a calcium/calmodulin dependent enzyme that is exclusively expressed in adult skeletal muscle. MYLK2 has been proposed to participate in signaling pathways (calcium signaling pathway, focal adhesion, regulation of actin cytoskeleton) and cellular processes (neuromuscular synaptic transmission, protein/amino acid phosphorylation). MYLK2 is involved in multiple molecular functions as a result of various subdomains that participate in ATP binding, calmodulin binding, nucleotide binding, protein serine/threonine kinase activity and transferase activity).
• Form: Recombinant protein stored in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.2mM DTT, 25% glycerol.
• Bio-activity: 270 nmol/min/mg
• Molecular Mass: ~74kDa
• Purity: >75%
• Applications: Kinase Assay, Western Blot
• Storage: Store at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. Avoid freeze/thaw cycles.
• Concentration: 0.1μg/μl

Gene Information

• Gene Name: MYLK2 myosin light chain kinase 2 [ Homo sapiens ]
• Official Symbol: MYLK2
• Synonyms: MYLK2; myosin light chain kinase 2; myosin light chain kinase 2, skeletal muscle; myosin light chain kinase 2, skeletal/cardiac muscle; KMLC; MLCK2; skeletal muscle myosin light chain kinase; skMLCK; MLCK
• Gene ID: 85366
• mRNA Refseq: NM_033118
• Protein Refseq: NP_149109
• MIM: 606566
• UniProt ID: Q9H1R3
• Chromosome Location: 20q13.31
• Pathway: Calcium signaling pathway, organism-specific biosystem; Calcium signaling pathway, conserved biosystem; Focal Adhesion, organism-specific biosystem; Focal adhesion, organism-specific biosystem; Focal adhesion, conserved biosystem; Gastric acid secretion, organism-specific biosystem; Gastric acid secretion, conserved biosystem
• Function: ATP binding; calmodulin binding; calmodulin binding; calmodulin-dependent protein kinase activity; myosin light chain binding; myosin light chain kinase activity; myosin light chain kinase activity; nucleotide binding; protein binding