Active Recombinant Human MUC16 Protein, Fc-Avi-tagged, Biotinylated

• Cat.No.: MUC16-43H
• Product Overview: Biotinylated Human CA125, Fc Tag, Avi Tag is expressed from HEK293 cells. It contains AA Gly 12660 - Met 12923 (Accession # Q8WXI7-1). This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag.

Specification

• Source: HEK293
• Species: Human
• Tag: Fc&Avi
• Predicted N Terminal: Gly 12660
• Form: Lyophilized from 0.22 um filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5, 10% trehalose.
• Bio-activity: Immobilized Human Mesothelin, His Tag at 10 ug/mL (100 uL/well) can bind Biotinylated Human CA125, Fc Tag, Avi Tag with a linear range of 2-78 ug/mL.
• Molecular Mass: The protein has a calculated MW of 56.9 kDa. The protein migrates as 75-100 kDa under reducing condition (SDS-PAGE) due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Characteristic: Biotinylation of this product is performed using Avitag technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin. The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: For long term storage, the product should be stored at lyophilized state at -20ºC or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8ºC for 12 months in lyophilized state; -70ºC for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.
• Protein length: 12660-12923 a.a.

Gene Information

• Gene Name: MUC16 mucin 16, cell surface associated [ Homo sapiens ]
• Official Symbol: MUC16
• Synonyms: MUC16; mucin 16, cell surface associated; mucin-16; CA125; FLJ14303; CA-125; MUC-16; CA125 ovarian cancer antigen; ovarian carcinoma antigen CA125; ovarian cancer-related tumor marker CA125
• Gene ID: 94025
• mRNA Refseq: NM_024690
• Protein Refseq: NP_078966
• MIM: 606154
• UniProt ID: Q8WXI7