Recombinant Human MUC16 protein(Asp12783-Ser13467), His-tagged

• Cat.No.: MUC16-10H
• Product Overview: Recombinant Human MUC16 protein(Asp12783-Ser13467)(Q8WXI7) is expressed from HEK293 with His tag at the C-Terminus.

Specification

• Source: HEK293
• Species: Human
• Tag: His
• Protein length: 12660-12923 a.a.
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human CA125, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human MSLN, hFc Tag with the EC50 of 7.8ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 76.17 kDa. Due to glycosylation, the protein migrates to 90-140 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE
  > 95% as determined by HPLC
• Storage: -20 to -80°C for 12 months as supplied from date of receipt.
  -80°C for 3-6 months after reconstitution.
  2-8°C for 2-7 days after reconstitution.
  Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: MUC16 mucin 16, cell surface associated [ Homo sapiens ]
• Official Symbol: MUC16
• Synonyms: MUC16; mucin 16, cell surface associated; mucin-16; CA125; FLJ14303; CA-125; MUC-16; CA125 ovarian cancer antigen; ovarian carcinoma antigen CA125; ovarian cancer-related tumor marker CA125
• Gene ID: 94025
• mRNA Refseq: NM_024690
• Protein Refseq: NP_078966
• MIM: 606154
• UniProt ID: Q8WXI7