Active Recombinant Human MOS Protein, GST-tagged

• Cat.No.: MOS-5484H
• Product Overview: Human MOS (NP_005363.1, 1 a.a. - 346 a.a.) full-length recombinant protein with GST tag expressed in baculovirus infected Sf21 cells.

Specification

• Species: Human
• Source: Insect Cells
• Tag: GST
• Description: MOS is a serine/threonine kinase that activates the MAP kinase cascade through direct phosphorylation of the MAP kinase activator MEK (MAP2K1; MIM 176872) (Prasad et al., 2008 [PubMed 18246541]).[supplied by OMIM
• Form: Liquid
• Bio-activity: The activity was determined by ELISA. The enzyme was incubated with GST-fused substrate protein, and after stopping kinase reaction by EDTA, the reaction solution was transferred into glutathione-coated plate. Phosphorylation was detected by anti-phospho antibody and HRP-labeled anti-rabbit IgG(or HRP-labeled anti-mouse IgG).
  Substrate: MAP2K1 [inactive mutant].
  ATP: 100 μM.
• Molecular Mass: 65 kDa
• Purity: 77 % by SDS-PAGE/CBB staining
• Applications: Functional Study
  SDS-PAGE
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol)

Gene Information

• Gene Name: MOS v-mos Moloney murine sarcoma viral oncogene homolog [ Homo sapiens ]
• Official Symbol: MOS
• Synonyms: MOS; v-mos Moloney murine sarcoma viral oncogene homolog; proto-oncogene serine/threonine-protein kinase mos; c-mos; proto-oncogene c-Mos; oocyte maturation factor mos; oncogene MOS, Moloney murine sarcoma virus; MSV; MGC119962; MGC119963
• Gene ID: 4342
• mRNA Refseq: NM_005372
• Protein Refseq: NP_005363
• MIM: 190060
• UniProt ID: P00540