Active Recombinant Human MMP12 Protein

• Cat.No.: MMP12-1134H
• Product Overview: Recombinant Human MMP12 Protein (Leu17-Cys470) with Val97Leu substitution was expressed in mouse myeloma cell line, NS0-derived.

Specification

• Species: Human
• Source: Mouse myeloma cell
• ProteinLength: 17-470 a.a.
• Description: This gene encodes a member of the peptidase M10 family of matrix metalloproteinases (MMPs). Proteins in this family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. The encoded preproprotein is proteolytically processed to generate the mature protease. This protease degrades soluble and insoluble elastin. This gene may play a role in aneurysm formation and mutations in this gene are associated with lung function and chronic obstructive pulmonary disease (COPD). This gene is part of a cluster of MMP genes on chromosome 11.
• Form: Pro form
• Bio-activity: Measured by its ability to cleave the fluorogenic peptide substrate, Mca-PLGL-Dpa-AR-NH2. The specific activity is >500 pmol/min/μg, as measured under the described conditions.
• Molecular Mass: 52 kDa
• N-terminal Sequence Analysis: Leu17
• Endotoxin: < 0.10 EU/μg of the protein by the LAL method.
• Purity: > 95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  6 months from date of receipt, -20 to -70 centigrade as supplied.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in MES, NaCl, CaCl2, CHAPS, ZnSO4 and PEG.
• Reconstitution: Reconstitute at 0.25 mg/mL in 10 mM MES, 0.1 M NaCl, 100 μM CaCl2, 0.1% CHAPS, 1 μM ZnSO4 and 0.1% PEG., pH 6.0.
• Shipping: The product is shipped with polar packs.

Gene Information

• Gene Name: MMP12 matrix metallopeptidase 12 [ Homo sapiens (human) ]
• Official Symbol: MMP12
• Synonyms: MMP12; matrix metallopeptidase 12; macrophage metalloelastase; matrix metallopeptidase 12 (macrophage elastase); matrix metalloproteinase 12 (macrophage elastase); EC 3.4.24.65
• Gene ID: 4321
• mRNA Refseq: NM_002426
• Protein Refseq: NP_002417
• MIM: 601046
• UniProt ID: P39900