Active Recombinant Human MINK1, GST-tagged

• Cat.No.: MINK1-1431H
• Product Overview: Recombinant human MINK1 (1-320) was expressed by baculovirus in Sf9 insect cells using a N-terminal GST tag.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: GST
• Protein Length: 1-320 aa
• Description: MINK1 is a member of the germinal center family of kinases that are homologous to the Ste20 family and regulate a wide variety of cellular processes, including cell morphology, cytoskeletal rearrangement, and survival. Overexpression of kinase-dead mutants of MINK1 leads to enhanced cell spreading, actin stress fiber formation, adhesion to extracellular matrix and decreased cell motility and invasion. MINK is activated after Ras induction via a mechanism involving reactive oxygen species and mediates stimulation of p38 MAPK downstream of the Raf/ERK pathway.
• Form: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.
• Bio-activity: 19 nmol/min/mg
• Molecular Mass: ~61 kDa
• Purity: >90%
• Applications: Kinase Assay, Western Blot
• Storage: Store at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. Avoid freeze/thaw cycles.
• Concentration: 0.1μg/μl

Gene Information

• Gene Name: MINK1 misshapen-like kinase 1 [ Homo sapiens ]
• Official Symbol: MINK1
• Synonyms: MINK1; misshapen-like kinase 1; misshapen like kinase 1 (zebrafish); B55; MAP4K6; MINK; misshapen/NIK related kinase; YSK2; ZC3; MEKKK 6; MEK kinase kinase 6; GCK family kinase MINK; misshapen/NIK-related kinase; MAPK/ERK kinase kinase kinase 6; serine/threonine protein kinase; mitogen-activated protein kinase kinase kinase kinase 6; hMINK; hMINKbeta; MGC21111
• Gene ID: 50488
• mRNA Refseq: NM_001024937
• Protein Refseq: NP_001020108
• MIM: 609426
• UniProt ID: Q8N4C8
• Chromosome Location: 17p13.2
• Pathway: Insulin Signaling, organism-specific biosystem; MAPK signaling pathway, organism-specific biosystem
• Function: ATP binding; nucleotide binding; protein binding; protein kinase activity; protein serine/threonine kinase activity; small GTPase regulator activity