Active Recombinant Human Methionine Sulfoxide Reductase B3, His-tagged

• Cat.No.: MSRB3-332H

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: MSRB3 (Methionine-R-sulfoxide reductase, EC1.8.4.6; accession number AAH40053) is one of three different mammalian MSRB enzymes. It carries out the reduction of methionine-R-sulfoxide to methionine. Human studies have shown the highest levels of expression in smooth muscle tissues. Its proposed function is the repair of oxidative damage to proteins to restore biological activity. MW = 22.5 kDa.
• Form: Liquid. Supplied in 0.1M TRIS-HCl pH 7.4 and 33% glycerol.
• Purity: >90% by SDS-PAGE.
• Activity assay: MetO-containing peptides are reduced by the enzyme in the presence of DTT.
• Activity: 1 nmol of hMSRB3 will reduce 2 nmol peptide-bound Met-S-sulfoxide in 1 min at 37°C.
• Usage: For in vitro use only.
• Storage: Quality guaranteed for 12 months store at - 80°C. Avoid freeze / thaw cycles.

Gene Information

• Gene Name: MSRB3 methionine sulfoxide reductase B3 [ Homo sapiens ]
• Synonyms: MSRB3; methionine sulfoxide reductase B3; FLJ36866; DKFZp686C1178; EC 1.8.4.-; Methionine-R-sulfoxide reductase B3, mitochondrial; MsrB3
• Gene ID: 253827
• mRNA Refseq: NM_001031679
• Protein Refseq: NP_001026849
• MIM: 14785
• UniProt ID: Q8IXL7
• Chromosome Location: 12q14.3
• Function: metal ion binding; oxidoreductase activity; peptide-methionine-(S)-S-oxide reductase activity; protein-methionine-R-oxide reductase activity IDA PubMed zinc ion binding