Active Recombinant Human MADCAM1 protein, Fc-Avi-tagged, Biotinylated

• Cat.No.: MADCAM1-0486H
• Product Overview: Biotinylated Recombinant Human MADCAM1 protein(Gln19-Gln333), fused with Fc and Avi tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc&Avi
• ProteinLength: 19-333 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
• Bio-activity: Immobilized Human ITGA4&ITGB7 Heterodimer Protein, His Tag&Tag Free at 5 μg/mL (100 μL/well) can bind Biotinylated Human MAdCAM-1 Protein, Fc,Avitag with a linear range of 0.2-16 ng/mL.
• Molecular Mass: This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag)The protein has a calculated MW of 63 kDa. The protein migrates as 70-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Gln19-Gln333
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE. >95% as determined by SEC-MALS.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: MADCAM1 mucosal vascular addressin cell adhesion molecule 1 [ Homo sapiens ]
• Official Symbol: MADCAM1
• Synonyms: MADCAM1; mucosal vascular addressin cell adhesion molecule 1; mucosal addressin cell adhesion molecule 1; MACAM1; MAdCAM-1; hMAdCAM-1; mucosal addressin cell adhesion molecule-1
• Gene ID: 8174
• mRNA Refseq: NM_130760
• Protein Refseq: NP_570116
• MIM: 102670
• UniProt ID: Q13477