Active Recombinant Human LTK protein, His-tagged

• Cat.No.: LTK-189H
• Product Overview: Recombinant Human LTK(Ser20-Pro424) fused with His tag at C-terminal was expressed in NS0.

Specification

• Species: Human
• Source: Mammalian Cells
• Tag: His
• Protein Length: 20-424 a.a.
• Description: LTK, also known as Leukocyte tyrosine kinase receptor and protein tyrosine kinase 1, is a type I transmembrane protein and shares a high degree of sequence similarity with kinases in the insulin receptor family. Alternative splicing generates several isoforms of 50-100 kDa. LTK contains a 408 aa extracellular domain, 25 aa transmembrane domain, and 415 aa cytoplasmic domain. The cytoplasmic region has several phosphotyrosines that interact with downstream signaling molecules. LTK is reported to undergo dimerand trimerization when under certain conditions. The extracellular domain of human LTK shares 75% homology with mouse LTK. It is expressed in preB lymphocytes, B lymphocytes, and other hematopoietic cells, as well as brain and placenta. Human LTK can bind to PLCgamma 1, PI3K, GAP and Raf1 in vivo. LTK promotes neurite outgrowth via PI3K/AKT and Ras/MAPK pathways. FAM150A and FAM150B can both bind to the extracellular domain of LTK, but only FAM150A is the specific ligand for LTK.
• Predicted N Terminal: Ser20
• Form: Lyophilized from a 0.2 μm filtered solution in PBS.
• Bio-activity: Measured by its binding ability in a functional ELISA. When Recombinant Human LTK is immobilized at 2 μg/mL, 100 μL/well, the concentration of Recombinant Human FAM-150A that produces 50% of the optimal binding response is 40-240 ng/mL.
• Molecular Mass: Predicted Molecular Mass: 42 kDa
  SDS-PAGE: 56-84 kDa, reducing conditions
• Endotoxin: <0.1 EU per 1 μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Reconstitution: Reconstitute at 500 μg/mL in PBS.

Gene Information

• Gene Name: LTK leukocyte receptor tyrosine kinase [ Homo sapiens ]
• Official Symbol: LTK
• Synonyms: LTK; leukocyte receptor tyrosine kinase; leukocyte tyrosine kinase; leukocyte tyrosine kinase receptor; TYK1; protein tyrosine kinase 1
• Gene ID: 4058
• mRNA Refseq: NM_001135685
• Protein Refseq: NP_001129157
• MIM: 151520
• UniProt ID: P29376