Active Recombinant Human LRP6 Protein, Fc Chimera

Source :	Mammalian cells
Species :	Human
Tag :	Fc
Predicted N Terminal :	Ala20
Form :	Lyophilized from a 0.2 µm filtered solution in PBS.
Bio-activity :	Measured by its binding ability in a functional ELISA.rhLRP-6/Fc Chimera binds rhDkk-1, biotin with an apparent KD < 25 nM.Biotinylated rmWnt-3a immobilized on a streptavidin-coated plate at 1 µg/mL can bind rhLRP-6/Fc Chimera with a linear range of 0.2 - 10 µg/mL. Optimal concentrations should be determined by each laboratory for each application.
Molecular Mass :	Recombinant Human LRP6, Fc Chimera has a calculated MW of 180 kDa (monomer). In SDS-PAGE migrates as 200 kDa, reducing conditions.
Purity :	>80%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage :	Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Reconstitution :	Reconstitute at 10 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Gene Name	LRP6 low density lipoprotein receptor-related protein 6 [ Homo sapiens ]
Official Symbol	LRP6
Synonyms	LRP6; low density lipoprotein receptor-related protein 6; low-density lipoprotein receptor-related protein 6; LRP-6; ADCAD2; FLJ90062; FLJ90421;
Gene ID	4040
mRNA Refseq	NM_002336
Protein Refseq	NP_002327
MIM	603507
UniProt ID	O75581
Chromosome Location	12p13.2