Active Recombinant Human LILRB4 protein, His-Avi-tagged, Biotinylated

• Cat.No.: LILRB4-309HB
• Product Overview: Biotinylated Recombinant Human LILRB4 protein(AAH26309)(Gln22-His257), fused with His tag and Avi tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: His&Avi
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Anti-LILRB4 Antibody, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human LILRB4, His Tag with the EC50 of 7.5ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 28.6 kDa. Due to glycosylation, the protein migrates to 38-42 kDa based on Tris-Bis PAGE result.
• Protein length: Gln22-His257
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE
  > 95% as determined by HPLC
• Storage: -20 to -80°C for 12 months as supplied from date of receipt.
  -20 to -80°C for 3-6 months in unopened state after reconstitution.
  2-8°C for 2-7 days after reconstitution.
  Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: LILRB4 leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 4 [ Homo sapiens ]
• Official Symbol: LILRB4
• Synonyms: HM18; ILT3; LIR5; CD85K; LIR-5; LILRB5
• Gene ID: 11006
• mRNA Refseq: NM_006847.3
• Protein Refseq: NP_006838.3
• MIM: 604821
• UniProt ID: Q8NHJ6