Active Recombinant Human Leptin

• Cat.No.: LEP-81H
• Product Overview: Recombinant Human leptin sequence (containing the signal peptide sequence, and the mature human leptin sequence) and was expressed in modifiedhuman 293 cells.

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Description: Leptin, also known as obesity factor and obese protein, is a member of the adipocytokine family that are cytokines expressed from adipose tissues. Other members of the adipocytokine family include TNF alpha, IL-6 and resistin. The general function of these molecules is regulation of food intake energy homeostasis, hematopoiesis, inflammation and immunity.
• Amino Acid Sequence: VPIQKVQDDTKTLI KTIVTRINDISHTQ SVSSKQKVT GLDFIPGLHP ILTLSK MDQT LAV YQQILTSMPSRNV IQISNDLENLRDLLH VLAFSKSCHLPWAS GLETL DSLGG VLEA SGYSTEVVALSRLQ GSLQDMLWQLDLSPGC.
• Molecular Mass: Under reducing conditions Leptin migrates as a band at approximately 15-16 kDa in SDS-PAGE. This compares with unmodified Leptin that has a predicted monomeric molecular mass of 16 kDa.
• PI: Leptinseparates into a number of isoforms with a pI between 5.0 and 7.5 in 2D PAGE due to post-translational modifications, in particular glycosylation. This compares with the unmodified Leptin that has a predicted pI of 5.7.
• Purity: >95%, as determined by SDS-PAGE and visualized by silver stain.
• Formulation: When reconstituted in 0.5 ml sterile phosphate-buffered saline, the solution will contain 1% human serum albumin (HSA) and 10% trehalose.
• Reconstitution: It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial.
• Storage: Lyophilized products should be stored at 2 to 8°C. Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
• Activity: The ED₅₀of Leptin is typically 0.25-0.35 ng/ml as measured in a cytotoxicity assay using the human K562 cell line.

Gene Information

• Gene Name: LEP leptin [ Homo sapiens ]
• Synonyms: LEP; leptin; FLJ94114; OB; OBS; leptin (murine obesity homolog); leptin (obesity homolog, mouse); obese, mouse, homolog of; obesity factor; Obesity factor; Obese protein
• Gene ID: 3952
• mRNA Refseq: NM_000230
• Protein Refseq: NP_000221
• MIM: 164160
• UniProt ID: P41159
• Chromosome Location: 7q31.3
• Pathway: Cytokine-cytokine receptor interaction; Jak-STAT signaling pathway; Adipocytokine signaling pathway; Neuroactive ligand-receptor interaction
• Function: growth factor activity; hormone activity; peptide hormone receptor binding; protein binding