Active Recombinant Human LAMA5 protein, Fc-tagged

• Cat.No.: LAMA5-0356H
• Product Overview: Recombinant Human LAMA5 protein(Glu2655-Ala3327(Lamininalpha5E8)&Asp1714-Leu1786(Lamininbeta1E8)&Asp1528-Pro1609(Laminingamma1E8)), fused with Fc tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: 2655-3327;1714-1786;1528-1609 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.
• Bio-activity: Immobilized Human ITGA6&ITGB1 Heterodimer Protein, His Tag&Tag Free at 5 μg/mL (100 μL/well) can bind Human Laminin 511 E8, Fc Tag with a linear range of 0.1-4 ng/mL.Immobilized Human ITGA3&ITGB1 Heterodimer Protein, His Tag&Tag Free at 5 μg/mL (100 μL/well) can bind Human Laminin 511 E8, Fc Tag with a linear range of 0.1-4 ng/mL.
• Molecular Mass: Human Laminin 511 E8, Fc Tag, produced by co-expression of Laminin alpha5E8, Laminin beta1E8 and Laminin gamma1E8, has a calculated MW of 76.7 kDa, 35.0 kDa and 35.7 kDa. The non-reducing (NR) protein migrates as 40-46 kDa (subunit Laminin beta1E8 and subunit Laminin gamma1E8) and 80 kDa (subunit Laminin alpha5E8) respectively due to glycosylation.
• Proteinlength: Glu2655-Ala3327(Lamininalpha5E8)&Asp1714-Leu1786(Lamininbeta1E8)&Asp1528-Pro1609(Laminingamma1E8)
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: LAMA5 laminin, alpha 5 [ Homo sapiens ]
• Official Symbol: LAMA5
• Synonyms: LAMA5; laminin, alpha 5; laminin subunit alpha-5; laminin alpha-5 chain; laminin-10 subunit alpha; laminin-11 subunit alpha; laminin-15 subunit alpha; KIAA1907
• Gene ID: 3911
• mRNA Refseq: NM_005560
• Protein Refseq: NP_005551
• MIM: 601033
• UniProt ID: O15230