Active Recombinant Human LAMA1 protein, Fc-tagged

• Cat.No.: LAMA1-0326H
• Product Overview: Recombinant Human LAMA1 protein(Phe1878-Gln2700(Lamininalpha1E8)&Leu1573-Gln1798(Lamininbeta2E8)&Asp1528–Pro1609(Laminingamma1E8)), fused with Fc tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: 1878-2700;1573-1798 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris,100 mM Glycine,25 mM Argine,150 mM NaCl,pH7.5 with trehalose as protectant.
• Bio-activity: Immobilized Human ITGA6&ITGB1 Heterodimer Protein, His Tag&Tag Free at 5 μg/mL (100 μL/well) can bind Human Laminin 121 E8, Fc Tag with a linear range of 0.5-62.5 ng/mL.Immobilized Human ITGA7X2B&ITGB1 Heterodimer Protein, His Tag&Tag Free at 5 μg/mL (100 μL/well) can bind Human Laminin 121 E8, Fc Tag with a linear range of 0.2-8 ng/mL.
• Molecular Mass: This protein carries a human IgG1 Fc tag at the N-terminusThe protein has a calculated MW of 92.2 kDa | 51.3 kDa | 35.6 kDa. The protein migrates as ＞90 kDa under non-reducing (NR) condition (SDS-PAGE).
• Proteinlength: Phe1878-Gln2700(Lamininalpha1E8)&Leu1573-Gln1798(Lamininbeta2E8)&Asp1528–Pro1609(Laminingamma1E8)
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE. >90% as determined by SEC-MALS.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: LAMA1 laminin, alpha 1 [ Homo sapiens ]
• Official Symbol: LAMA1
• Synonyms: LAMA1; laminin, alpha 1; LAMA; laminin subunit alpha-1; S-LAM alpha; laminin A chain; S-laminin subunit alpha; laminin-3 subunit alpha; S-LAM-alpha
• Gene ID: 284217
• mRNA Refseq: NM_005559
• Protein Refseq: NP_005550
• MIM: 150320
• UniProt ID: P25391