Active Recombinant Human Kringle Containing Transmembrane Protein 2, His-tagged

• Cat.No.: KREMEN2-5674H
• Product Overview: Recombinant Human KREMEN2protein was expressed in Murine myeloma cell line with a C-terminal 10-Histag. Gln19-Ala364.

Specification

• Species: Human
• Source: Mammalian Cells
• Tag: His
• ProteinLength: 19-364 a.a.
• Description: KREMEN2 proteins aretype I transmembrane proteins that contain extracellular kringle, WSC and CUBdomains and an intracellular region without any conserved motifs. Two relatedmembers, KREMEN1, and 2, have been identified. KREMEN bind a subset of thesecreted Dickkopf proteins (Dkk1, 2, and 4) with high affinity to modulatethe canonical Wnt signaling pathway that is transduced by the ternaryreceptor complex composed of Wnt, the seventransmembrane domain receptorFrizzled, and the LDLreceptorrelated protein 5/6 coreceptor.
• Form: Lyophilized from a0.2 μm filtered solution in PBS with BSA as a carrier protein.
• N-terminalSequence: Gln19 predicted
• Molecular Weight: 38 kDa
• Activity: Measuredby its binding ability in a functional ELISA. Immobilized rhKREMEN2/ his at 4μg/mL (100 μL/well) can bind rhDkk1 with an apparent KD < 10nM.
• Endotoxin Level: <1.0 EUper 1 μg of the protein by the LAL method.
• SDS-PAGE: 59-63kDa, reducing conditions.
• Purity: >95%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution: 100μg/mL in sterile PBS
• Storage: Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 12 months fromdate of receipt, -20 to -70°C as supplied. 1 month, 2 to 8°C under sterileconditions after reconstitution. 3 months, -20 to -70°C under sterileconditions after reconstitution.

Gene Information

• Gene Name: KREMEN2 kringle containingtransmembrane protein 2 [ Homo sapiens ]
• Official Symbol: KREMEN2
• Synonyms: KREMEN2; kringlecontaining transmembrane protein 2; KRM2; MGC10791; MGC16709; kremen protein2; dickkopf receptor 2; kringle domain-containing transmembrane protein 2;kringle-containing protein marking the eye and the nose; OTTHUMP00000159226;OTTHUMP00000159227
• Gene ID: 79412
• mRNA Refseq: NM_024507
• Protein Refseq: NP_078783
• MIM: 609899
• UniProt ID: Q8NCW0
• Chromosome Location: 16p13.11
• Pathway: Presenilin action inNotch and Wnt signaling; Wnt signaling network