Active Recombinant Human KLK4 protein, His-tagged

• Cat.No.: KLK4-1169H
• Product Overview: Recombinant Human KLK4 protein(NP_004908.3)(Met1-Ser254), fused with His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Met1-Ser254
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to cleave the fluorogenic peptide substrate Boc-VPR-AMC. The specific activity is >250 pmoles/min/μg.(Activation description: The proenzyme needs to be activated by Thermolysin for an activated form)
• Molecular Mass: The secreted recombinant human KLK4 consists of 239 amino acids and predictes a molecular mass of 25.8 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhKLK4 is approximately 30-35 kDa due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 94 % as determined by SDS-PAGE
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: KLK4 kallikrein-related peptidase 4 [ Homo sapiens ]
• Official Symbol: KLK4
• Synonyms: KLK4; kallikrein-related peptidase 4; kallikrein 4 (prostase, enamel matrix, prostate) , PRSS17; kallikrein-4; EMSP; EMSP1; KLK L1; PSTS; prostase; serine protease 17; kallikrein-like protein 1; androgen-regulated message 1; enamel matrix serine protease 1; enamel matrix serine proteinase 1; ARM1; AI2A1; KLK-L1; PRSS17; kallikrein; MGC116827; MGC116828
• Gene ID: 9622
• mRNA Refseq: NM_004917
• Protein Refseq: NP_004908
• MIM: 603767
• UniProt ID: Q9Y5K2